HomeFAQsHow can I verify successful amplification and specificity of my RCA reaction?
FAQ: How can I verify successful amplification and specificity of my RCA reaction?
RCA product quality and specificity can be assessed by digesting with a restriction enzyme and running the digested products on an agarose gel beside a DNA ladder such as Quick-Load® Purple 1 kb Plus DNA Ladder (NEB #N0550). Digested RCA products should show the same fragment sizes expected for the digested plasmid DNA template.
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