FAQ: How can I design my own i7 indexing/barcoding primer for additional barcoding options?

The NEBNext primer and adaptor sequences are similar to TrueSeq, therefore please use the below (i7) base sequence to design your own primers. Note that the “N’s” represent the place holder for your index design.


Please note you have to validate the primers experimentally. This kit is designed to work with the SR primer (i5) ) (NEB #E7310) component supplied with the kit.