Thermostable USER III Enzyme is 100% active in Thermopol Buffer and has limited activity in other buffers. Activity in NEBuffers at 65°C is as follows:
| NEBuffer | r1.1 | r2.1 | r3.1 | rCutSmart™ |
|---|---|---|---|---|
| % Activity | 100 | 50 | 50 | 75 |
Additionally, Thermolabile USER II Enzyme is 100% active in many polymerase and PCR buffers (see chart below).
| Buffer | Activity (Relative to Thermopol Buffer) |
|---|---|
| Thermopol II Reaction Buffer (NEB #B9004) | 100% |
| Q5® Reaction Buffer (NEB #B9027) | 50% |
| Colorless GoTaq® Flexi Buffer (Promega®) | 75% |
| PCR Buffer (Qiagen®) | 75% |
| iTaq Buffer | 100% |
| AmpliTaq Gold® 360 Buffer (ThermoFisher®) | 100% |
| Fast Start™ Taq DNA Pol PCR Buffer (Sigma Aldrich) | 0% |
| Ex Taq Buffer (Takara) | 0% |
| Dream Taq Buffer (ThermoFisher®) | 75% |
| Platinum II PCR Buffer (Invitrogen) | 75% |
- AmpliTaq Gold® and Thermo Fisher® are registered trademarks and property of Thermo Fisher Scientific.
- GoTaq® and Promega® are registered trademarks of Promega, Inc.
- Qiagen® is a registered trademark of Qiagen, Inc.
- Fast Start™ is a trademark of Sigma Aldrich.
