FAQ: Will Msz Exonuclease I work in other buffers?

Yes.  Most PCR buffers are compatible. No additional reaction buffer is necessary if the enzyme is added to a PCR reaction.

Incubation in the following buffers at 55°C for 15 minutes resulted in the relative activity listed:

 NEBuffer  % Activity
 r1.1  (NEB# B7030)  20
r2.1  (NEB# B6002  50
 r3.1  (NEB# B6003)  50
 rCutSmartTM  (NEB# B6004)  100
 Exonuclease I  Reaction Buffer (NEB# B0293)  10
 ThermoPol (NEB# B9004  90