FAQ: Are reaction conditions the same for lyophilized and liquid Luna mixes?

Yes, reaction conditions for the LyoPrime Luna® Probe One-Step RT-qPCR Mix with UDG (NEB #L4001) are the same as for our corresponding liquid product, the Luna Probe One-Step RT-qPCR 4X Mix with UDG (NEB #M3019). Both mixes include the engineered Luna WarmStart Reverse Transcriptase, which possesses higher thermostability than many other RTs and has an optimal reaction temperature of 55°C. Please see the protocol page (L4001 vial or L4001 plate) for additional details on reaction setup and cycling conditions.