HomeFAQsDo you recommend template-encoding the poly(A) tail or using E. coli Poly(A) Polymerase?
FAQ: Do you recommend template-encoding the poly(A) tail or using E. coli Poly(A) Polymerase?
Both methods will result in an mRNA with a poly(A) tail. However, using a template-encoded poly(A) tail will reduce the overall workflow time and result in a defined poly(A) tail length. If using Poly(A) Polymerase (NEB #M0276), we would recommend purifying the IVT reaction prior to the addition of the poly(A) tail. Using Poly(A) Polymerase will result in a range of tail lengths and an increased workflow time.
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