HomeFAQsHow can I determine the annealing temperature for my LunaScript Multiplex One-Step RT-PCR reactions?
FAQ: How can I determine the annealing temperature for my LunaScript Multiplex One-Step RT-PCR reactions?
Use of the NEB Tm Calculator is highly recommended for LunaScript Multiplex One-Step RT-PCR reactions as optimal annealing temperatures tend to be higher for Q5 Hot Start DNA Polymerase. When performing multiplex RT-PCR, use the annealing temperature calculated for the amplicon with the lowest annealing temperature.
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