FAQ: Can I reduce the reaction volume in the LunaScript Multiplex One-Step RT-PCR reactions?

Yes, for many targets, the reaction volume can be cut in half (from the standard 25 µl to 12.5 µl) without compromising the PCR yield. Simply scale down the reagent volume while using the same amount of RNA input. Further reducing the volume may pose a challenge for manual setup due to pipetting difficulties.