FAQ: Can I add random hexamers or oligo (dT) primers to LunaScript Multiplex One-Step RT-PCR reactions?

Using random hexamers or oligo (dT) primers with LunaScript One-Step RT-PCR reactions is not recommended. Inclusion of these primers may reduce the specificity of target amplification. Additionally, random hexamers require room temperature incubation for primer annealing, therefore, it is not compatible with Luna WarmStart Reverse Transcriptase, which requires full WarmStart activation at 50°C or higher. Instead, we recommend the use of gene-specific primers.