FAQ: Are the PCR products generated with the LunaScript Multiplex One-Step RT-PCR Kit compatible with a TA cloning system?

The PCR products created by Q5 Hot Start High-Fidelity DNA Polymerase contain blunt DNA ends and can be directly cloned using the NEB PCR Cloning Kit (NEB #E1202). A dA-tailing step is required for a TA-based cloning system.