FAQ: What components are included in the WarmStart Fluorescent LAMP/RT-LAMP Kit (with UDG)?

The kit includes a 2X Master Mix containing a reaction buffer with all necessary cofactors at optimized 2X concentrations for LAMP assays: dNTPs, MgSO4, Bst 2.0 WarmStart DNA Polymerase, WarmStart RTx, Antarctic Thermolabile Uracil DNA Glycosylase (UDG), and dTTP/dUTP. The kit also contains a 50X LAMP Fluorescent Dye that binds to dsDNA for real-time detection of LAMP. To perform a LAMP reaction, the user adds primers, target DNA or RNA and adjusts the Master Mix to a final concentration of 1X.  Detailed instructions can be found in the Kit Manual.