HomeFAQsCan I use a shorter incubation time for my cDNA synthesis reaction when using the LunaScript® RT Master Mix (Primer-free)? Does longer incubation increase the yield of cDNA?
FAQ: Can I use a shorter incubation time for my cDNA synthesis reaction when using the LunaScript® RT Master Mix (Primer-free)? Does longer incubation increase the yield of cDNA?
Under recommended conditions (55°C for 10 min), the LunaScript RT Master Mix (Primer-free) can make cDNA up to 9 kb. For some targets/workflows, products can be detected after 5 min. In some cases, longer incubation (up to 60 minutes) may increase cDNA yields. Pilot experiments can be performed to determine the optimal incubation time.
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