FAQ: For high Ct samples, how can the data throughput be increased?

For samples of Ct value 30 or higher, sequencing failures may increase. For such high Ct samples, two or more barcoding reactions (step 1.4.1) can be performed for each PCR amplicon, to increase the data throughput. We recommend use of the same barcode if the reactions are from the same sample, for easier downstream data analysis.