FAQ: What Ct values are recommended for inputs used with the NEBNext ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies)?

Consistent with guidelines from the ARTIC Network, viral RNA input from clinical samples should be between Ct 18-35. If Ct is between 12-15, it is recommended to dilute the sample 100-fold in water. If the Ct is between 15-18 a 10-fold dilution in water is recommended. This will reduce the likelihood of PCR inhibition.