HomeFAQsAre there tips to avoid barcode cross contamination?
FAQ: Are there tips to avoid barcode cross contamination?
To avoid barcode cross contamination or mismatch during base calling, it is recommended to run guppy again to sort reads with both 5′ and 3′ ligated with the same barcodes. Oxford Nanopore Technologies provides additional information on use of guppy with the option of basecall reads with dual barcodes.
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