FAQ: How do I make SARS-CoV-2 N gene mRNA from this positive control?

PacI (NEB #R0547) restriction digestion is recommended to linearize the plasmid, followed by DNA purification using the Monarch® PCR & DNA Cleanup Kit (NEB #T1030S). The DNA elution can be used to generate RNA by using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB #E2050S). To have enough DNA template to start with, propagation of the plasmid in advance is recommended.