FAQ: What targets do the SARS-CoV-2 Primer/Probe mix detect? How were these primers chosen?

The SARS-CoV-2 Primer/Probe mix provided with this kit contains primers and probes specific to two regions of the SARS-CoV-2 virus N gene (based on sequences provided by the CDC). However, they have been modified to contain different fluorophores (N1, HEX; N2, FAM) to enable simultaneous observation on two different channels of a real time instrument. To ensure the integrity of the input material and absence of inhibition, an optimized internal control (IC) primer and probe set, designed to amplify the human RNaseP gene, is also provided in the primer mix. Amplification of the IC is observed in the Cy5 channel.