Home FAQs Does the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit enable carryover prevention/contamination reduction?

FAQ: Does the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit enable carryover prevention/contamination reduction?

Yes, the kit features the Luna Probe One-Step RT-qPCR 4X Master Mix with UDG (NEB #M3019), which is formulated with a mixture of dTTP and dUTP. This enables the incorporation of dU into the reaction products without sacrificing amplification efficiency. Antarctic Thermolabile Uracil DNA glycosylase (UDG) in the mix digests RT-qPCR products containing dU, preventing contamination from carryover amplification products. 

Because RT-qPCR can generate significant quantities of DNA, best practices include the use of various strategies to reduce contamination, such as the use of environmental controls and not opening vessels post amplification.