Home FAQs What are the differences between the Luna One-Step RT-qPCR products (NEB #E3006, #E3007 and #M3019)?

FAQ: What are the differences between the Luna One-Step RT-qPCR products (NEB #E3006, #E3007 and #M3019)?

All three products are optimized for 1-step RT-qPCR by probe detection. NEB #M3019 is a one-tube 4X master mix containing everything for probe-based, one-step RT-qPCR detection except for templates, primers, and probes. The NEB #E3006/#E3007 products contain separate tubes for the 2X reaction mix and 20X enzyme mix. Also, the M3019 product includes not only dUTP, but also a thermolabile UDG, to fully enable carryover prevention. 
  NEB #M3019
Luna Probe One-Step RT-qPCR 4X Mix with UDG		 NEB #E3006
Luna Universal Probe One-Step RT-qPCR Kit		 NEB #E3007
Luna Probe One-Step RT-qPCR Kit (No ROX)
Format 4X Master Mix 2X Reaction Mix
20X Enzyme Mix		 2X Reaction Mix
20X Enzyme Mix
Carryover prevention included Y N
(requires UDG addition)		 N
(requires UDG addition)
Normalization dye
(universal ROX)		 Y Y N
Multiplexing Y (up to 5-plex) Y (up to 5-plex) Y (up to 5-plex)
Maximum sample volume
(20 µl RT-qPCR reaction)		 up to 10 µl up to 8 µl up to 8 µl
Standard cycling condition 25°C for 30 sec. (UDG treatment);
55°C for 10 min;
95°C for 1 min;
45 cycles of 95°C for 10 sec., 60°C for 30 sec.		 55°C for 10 min;
95°C for 1 min;
45 cycles of 95°C for 10 sec., 60°C for 30 sec.		 55°C for 10 min;
95°C for 1 min;
45 cycles of 95°C for 10 sec., 60°C for 30 sec.