FAQ: What is the difference between the WarmStart® Colorimetric LAMP 2X Master Mix with UDG (NEB #M1804) and the WarmStart Colorimetric LAMP 2X Master Mix (DNA & RNA) (NEB #M1800)?

Both WarmStart Colorimetric LAMP 2X Master Mixes contain a pH-sensitive dye in a low-buffer reaction solution that changes color from bright pink to yellow upon DNA amplification. This simple visual detection combined with a single temperature incubation makes it possible to use less sophisticated equipment (heat blocks, incubators, etc) than with typical LAMP reactions.

The WarmStart® Colorimetric LAMP 2X Master Mix with UDG is a modified version of M1800 that contains dUTP and Antarctic Thermolabile UDG (NEB #M0372), which prevents amplification of previous reaction products that contain uracil. These additions reduce the possibility of carryover contamination (where the product of a previous reaction can unexpectedly serve as the substrate of a subsequent reaction).

Both mixes contain WarmStart Bst 2.0® and WarmStart RTx and can be used to amplify both RNA and DNA/cDNA targets.