FAQ: How many sequencing reads per sample do you recommend using with the NEBNext Direct BRCA1/BRCA2 Panel?

The recommendation would depend on whether the application is for somatic or germline variant calling, and also the DNA input that is used. Using 100 ng of DNA input, with 200k reads, > 500X de-duplicated coverage is expected using Illumina® 2 x 75 bp sequencing. The same DNA input will archive 100X coverage with fewer than 50k sequencing reads.