FAQ: Following the NEBExpress cell-free protein synthesis reaction, how should I analyze the protein? Is it possible to use native SDS-PAGE or other functional assays to determine the activity of the expressed protein?

In vitro protein synthesis reactions produced by the NEBExpress Cell-free E coli Protein Synthesis System can be directly loaded onto either native or denaturing SDS-PAGE gels without the need for acetone or TCA precipitation. Reactions can also be directly tested, without protein purification, in functional assays (enzymatic, colorimetric, fluorescent, gel based, etc.), as long as the protein synthesis components (nucleotides, amino acids, buffer) do not interfere with the specific assay measurements.