FAQ: Do NEB Stable Competent E.coli require an outgrowth recovery period after transformation with AmpR plasmids?

Transformation reactions with AmpR plasmids do not require an outgrowth period after addition of Outgrowth Medium. Plasmid selection using antibiotics other than ampicillin requires an outgrowth period of 60 minutes at 30°C before plating on selective media. 30°C or 37°C  may be used for plate incubation, however 30°C is recommended as some constructs may be unstable at elevated temperatures.