FAQ: What is known about binding in the presence of nonionic detergents?

Some fusion proteins do not bind efficiently (< 5% binding) in the presence of 0.2% Triton X–100 or 0.25% Tween-20, while other fusions are unaffected. For one fusion that does not bind in 0.25% Tween- 20, diluting the Tween-20 to 0.05% typically restores about 80% of the binding.