FAQ: Is NudC decapping activity affected by the structure of the RNA?

Yes. It has been reported that NudC's rate of decapping is slower on RNA containing 5′ secondary structure (1). If the RNA is expected to contain substantial secondary structure, one can increase the enzyme concentration and/or increase the incubation time to achieve a higher level of decapping.



1. Höfer K. et al. (2016) Nat Chem Biol.12, 730-734.