FAQ: Is NudC decapping activity affected by 2′O methylation of the +1 nt?

It has been reported that NudC decapping activity is blocked by 2′O methylation of the +1 nt in case of NAD+, i.e., nicotinamide diphosphate 2′O-Me-adenosine (1). On the other hand, NEB has found that cap 1 RNA (m7G-capped RNA with 2'O methylation at +1 A or G) is not significantly decapped by NudC.


1. Mlynarska-cieslak, A., et al. (2018) Org. Lett. 20, 7650-7655.