FAQ: Do I need to purify my plasmid or linear DNA template prior to synthesis?

Template purity is important for successful in vitro transcription/translation; we recommend the Monarch® Plasmid Miniprep Kit (NEB #T1010) or Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB #T1030) to isolate plasmid or linear DNA.  In addition, we recommend using a DNA template concentration ≥100 ng/µl.