HomeFAQsCan I express large proteins with NEBExpress Cell-free E. coli Protein Synthesis System?
FAQ: Can I express large proteins with NEBExpress Cell-free E. coli Protein Synthesis System?
The NEBExpress Cell-free E. coli Protein Synthesis System can produce high molecular weight targets due to stabilization of the mRNA template in a low RNase environment. Proteins from 17 to 230 kDa have been successfully synthesized in an active form (unpublished results). For example, a functionally active 230 kDa protein, β-galactosidase from Streptococcus, was expressed using the NEBExpress Cell-free E. coli Protein Synthesis System.
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