Home FAQs What cell lines are compatible with the Luna Cell Ready Probe One-Step RT-qPCR Kit (NEB #3031)?

FAQ: What cell lines are compatible with the Luna Cell Ready Probe One-Step RT-qPCR Kit?

Numerous cell lines can be used for direct RNA detection using the Luna Cell Ready One-Step RT-qPCR Kit. However, some cell lines may contain high levels of inhibitors that may affect cell lysis or the RT-qPCR reaction. Culture medium and drug/RNAi/CRISPR treatment can also affect cell growth status and levels of inhibitors.

 The table below highlights the results observed with various mammalian cell lines using a 5-log dilution series (100,000 -10 cells per 50 µl lysis), and subsequent detection of 1 µl of lysate in a 20 µl one-step RT-qPCR experiment. For each cell line, linear results are indicated in the final column of the table below. In addition to these mammalian cell lines, the kit is also compatible with insect cell lines. For cell lines not listed below, it is recommended to perform a pilot test for the optimal cell input numbers for lysis as well as the amount of lysate that can be carried into the RT-qPCR.

 
Cell lines Property Species Cell number per 50 μl lysate
HeLa Adherent Hs. Cervix, adenocarcinoma 10-100,000
A549 Adherent Hs. Lung, carcinoma 10-100,000
Jurkat Suspension Hs. T lymphocyte, leukemia 10-100,000
HEK293 Adherent Hs. Kidney 10-100,000
U2Os Adherent Hs. Bone, osteosarcoma 10-100,000
HepG2 Adherent Hs. Liver, carcinoma 10-1,000
K-562 Suspension Hs. Lymphoblast, leukemia 10-100,000
NCI-H460 Adherent Hs. Lung, carcinoma 10-100,000
SK-N-SH Adherent Hs. Brain, Neuroblastoma 10-10,000