FAQ: When using cell lysates generated from Luna Cell Ready Lysis Module, can RT-qPCR experiments be set up at room temperature?

Yes, RT-qPCR can be set up at room temperature. However, cell lysates are sensitive to temperature; if possible, keep the lysates on ice during reaction setup. Once the RT-qPCR plate is set up, run the reactions as soon as possible. If short term storage of the RT-qPCR plate is required, keep at 4°C for no longer than 5 hours for dye-based detection. For probe-based detection, RT-qPCR experiment should proceed immediately after setup. For high throughput screening using automation, minimize the time that lysates sit at room temperature (no longer than 15 min prior to starting process), and avoid freeze and thaw cycles.