Numerous cell lines can be used for direct RNA detection using the Luna Cell Ready One-Step RT-qPCR Kit. However, some cell lines may contain high levels of inhibitors that may affect cell lysis or the RT-qPCR reaction. Culture medium and drug/RNAi/CRISPR treatment can also affect cell growth status and levels of inhibitors.
The table below highlights the results observed with various mammalian cell lines using a 5-log dilution series (100,000 -10 cells per 50 µl lysis), and subsequent detection of 1 µl of lysate in a 20 µl one-step RT-qPCR experiment. For each cell line, linear results are indicated in the final column of the table below. In addition to these mammalian cell lines, the kit is also compatible with insect cell lines. For cell lines not listed below, it is recommended to perform a pilot test for the optimal cell input numbers for lysis as well as the amount of lysate that can be carried into RT-qPCR.
|
Cell lines |
Property |
Species |
Cell number per 50 μl lysate |
|---|---|---|---|
|
HeLa |
Adherent |
Hs. Cervix, adenocarcinoma |
10 - 100,000 |
|
A549 |
Adherent |
Hs. Lung, carcinoma |
10 - 100,000 |
|
Jurkat |
Suspension |
Hs. T lymphocyte, leukemia |
10 - 100,000 |
|
HEK293 |
Adherent |
Hs. Kidney |
10 - 100,000 |
|
U2Os |
Adherent |
Hs. Bone, osteosarcoma |
10 - 100,000 |
|
HepG2 |
Adherent |
Hs. Liver, carcinoma |
10 - 1,000 |
|
K-562 |
Suspension |
Hs. Lymphoblast, leukemia |
10 - 100,000 |
|
NCI-H460 |
Adherent |
Hs. Lung, carcinoma |
10 - 100,000 |
|
SK-N-SH |
Adherent |
Hs. Brain, Neuroblastoma |
10 - 10,000 |
