Home FAQs Is cell washing prior to lysis necessary?

FAQ: Is cell washing prior to lysis necessary?

For best results, we recommend washing cells to get rid of unwanted media and dead cells. However, in situations where this is not practical, such as high throughput screening or extremely sensitive gene expression studies where cell washes may change RNA abundance, plate flipping can be used for effective medium removal. If the washing step is omitted, lysates should be used immediately, without freeze and thaw cycles.