FAQ: How much cell lysate should I use as input? (NEB #3030)

The amount of cell lysate that can be added to one-step RT-qPCR will depend on the RT-qPCR kit. When using the Luna Universal One-Step RT-qPCR Kit, lysate can easily comprise up to 10% of total reaction volume and ≤ 4,000-cells per 20 μl RT-qPCR experiment.