HomeFAQsWhy is imidazole not necessary in the lysis/binding buffer? Why does the wash buffer contain only 5mM imidazole?
FAQ: Why is imidazole not necessary in the lysis/binding buffer? Why does the wash buffer contain only 5mM imidazole?
NEBExpress™ Ni Spin Columns utilize a highly selective, proprietary resin that unlike conventional Ni-NTA, requires less imidazole in the purification. Nickel ions are immobilized on the matrix surface via a proprietary ligand that is highly uniform and stable and offers excellent resistance to a wide range of chemicals, including NaOH, EDTA, DTT and β-ME.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.
