FAQ: Does Tte UvrD Helicase (NEB #M1202) require any specific DNA structure for unwinding?

No, Tte UvrD Helicase can utilize any dsDNA structure as a substrate for unwinding. This includes duplex DNA and duplexes with 3′and 5′ ssDNA overhangs (1-3). 

References

1. An, L., Tang, W., Ranalli, T.A., Kim, H.J., Wytiaz, J., and Kong, H. (2005). J Biol Chem 280(32):28952-8.
2. Mauris, J., and Evans, T.C. Jr. (2010). J Biol Chem 285(15):11087-92.
3. Vincent, M., Xu, Y., and Kong, H. (2004). EMBO Rep 5(8):795-800.