FAQ: Do I need to add ATP for Tte UvrD Helicase activity?

If using Tte UvrD Helicase for helicase activity independent from any DNA polymerase or amplification activity, or in Isothermal Amplification Buffer, then yes, 1 mM ATP should be added to the reaction mixture. If adding Tte UvrD Helicase to an amplification reaction (for example, to WarmStart LAMP Master Mix) then the dATP present in the mixture will be utilized for unwinding.