HomeFAQsMy purification failed and/or why is my yield lower than expected?
FAQ: My purification failed and/or why is my yield lower than expected?
Multiple reasons may account for purifications resulting in low yield. It is important to first optimize the expression level of the soluble target protein by altering induction time/ temperature and level of inducer. It is possible that the protein has formed inclusion bodies or the tag has been sequestered and therefore is unable to bind to the beads. In this case, the purification should be performed under denaturing conditions. Insufficient cell lysis and extraction will also lead to reduced yield; it is important to ensure your lysis procedure is sufficient. In some cases, extending the binding time may improve protein binding.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.
