HomeFAQsIs T7 expression subject to catabolite repression in BL21(DE3) and derivative strains?
FAQ: Is T7 expression subject to catabolite repression in BL21(DE3) and derivative strains?
The lambda DE3 prophage carries the T7 RNA polymerase gene (T7gene1). Expression of the T7 RNA Polymerase is controlled by the lacUV5 promoter. Therefore, glucose addition will result in catabolite repression and basal protein expression from T7 promoter vectors will be better controlled when glucose is present in the media. When glycerol is the primary carbon source, there should be no effect on basal T7 expression.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.
