FAQ: What are the main causes of reaction failure when using Hi-T7 RNA Polymerase?

The purity of the DNA template is very important and should be prepared by purification methods such as spin column or phenol:chloroform extraction. In addition, to avoid RNase contamination we recommend wearing gloves and using filter tips and RNase-free tubes.

It is important to mix the reaction components well after thawing and to set the reaction up at room temperature in the order listed in the protocol for optimal yield.