FAQ: At what temperature is Hi-T7 RNA Polymerase active?

Hi-T7 RNA Polymerase is active between 37°C and 56°C. When performing transcription reactions at temperatures higher than the suggested 50°C, it is recommended to limit the reaction time to 30 minutes or less as incubation of RNAs at high temperature for prolonged periods in the presence of MgCl2 (a component of the Hi-T7 RNA Polymerase Reaction Buffer) may lead to RNA degradation.