FAQ: What cell types does the NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module support?

The module is optimized for use with a variety of eukaryotic cell types. However, the cell lysis is not compatible with fixed cells or cells with cell walls (e.g., plant and fungal cells). If good quality RNA is extracted from these samples, Section 2 of the product manual for low input RNA can be followed for cDNA synthesis, amplification and library generation. The module is not compatible with mRNA lacking poly(A) tails (e.g., from bacterial cells).