FAQ: Can I resuspend the cells in buffers/medium other than PBS before cell collection?

We strongly recommend that cells be resuspended in PBS buffer before cell collection. If PBS alone is not a viable option, cells can be resuspended in PBS supplemented with BSA. Up to 0.5 ng of BSA can be included in the cDNA Synthesis and Amplification reaction. If cells are resuspended in cell culture medium, for example, DMEM with 10% FBS, the carry-over volume during cell collection should be minimal (10-15 nl). For sorted cells do not use more than 5 µl carryover volume. Please see this FAQ.