HomeFAQsWhat is the difference between EnGen Cas9 Nickase NLS, S. pyogenes (M0650) and EnGen Spy Cas9 NLS (M0646)?
FAQ: What is the difference between EnGen® Spy Cas9 Nickase (NEB #M0650) and EnGen Spy Cas9 NLS (NEB #M0646)?
EnGen Spy Cas9 Nickase contains a single point mutation (D10A) that inactivates the RuvC nuclease domain, but leaves the HNH nuclease domain intact, rendering the enzyme a nickase. EnGen Spy Cas9 NLShas both nuclease domains active and is able to cleave both strands of dsDNA.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.
