FAQ: How has MspJI been used for epigenetic studies?

This enzyme has been used for epigenetic analysis in which target DNA containing CpG sites methylated in both strands is cleaved by MspJI to generate 32 bp fragments containing the methylated CpG sites. The isolated 32 bp fragments can be sequenced to reveal 5-mC modification sites (Cohen-Karni et al., (2011) PNAS 108: 11040-11045).

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