FAQ: How long should I incubate the Colorimetric LAMP reaction before checking the results?

Our recommended incubation time for colorimetric LAMP is 30 min. At this end point, most positive LAMP reactions will have changed color and non-template controls will be negative. If desired, the time can be shortened to 15–20 minutes with good assays or high copy targets, or lengthened to up to 60 minutes with very low input, impure samples, and slower assays. If optimizing reaction time, progress can be simply checked by looking at the reaction colors and incubating longer if incomplete. Note that when extending the reaction time, NTCs must also be monitored to ensure that false positives are not present.