Home FAQs The protocol for Instant Sticky-end Ligase Master Mix indicates the reaction should not be heat inactivated. How can I inactivate the ligation activity?

FAQ: The protocol for Instant Sticky-end Ligase Master Mix indicates the reaction should not be heat inactivated. How can I inactivate the ligation activity?

There is typically no need to inactivate Sticky-end ligation reactions. The reaction may be used directly for transformation with chemically competent cells, or the DNA can be cleaned up by ethanol precipitation, gel purification, or any suitable DNA purification column or beads to separate the ligation product from the enzyme activity and other reaction components.

If you desire to halt the reaction without cleanup, you can add an equal volume of 50 mM EDTA and mix by pipetting, however, this will interfere with downstream reactions requiring magnesium or other divalent cations.