HomeFAQsCan PNGase A be used under non-denaturing (native) conditions?
FAQ: Can PNGase A be used under non-denaturing (native) conditions?
No, PNGase A requires that the glycoprotein be at least mildly reduced prior to deglycosylation. We recommend using PNGase A with only DTT at a final concentration of 40 mM. The reaction should be heated at 55°C for 10 minutes and then cooled before adding the PNGase A. Increasing the amount of PNGase A or the length of incubation at 37°C may be necessary to achieve full deglycosylation.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.
