Home FAQs How much Endo F3 should I use to deglycosylate a glycoprotein under native conditions?

FAQ: How much Endo F3 should I use to deglycosylate a glycoprotein under native conditions?

Endo F3 should always be used under native conditions. We recommend using 1µl of Endo F3 per 20µg of native glycoprotein.  Optimal incubation times and enzyme concentrations must be determined empirically for a particular substrate.  Typical reaction conditions are as follows:
  1. Combine 20 µg of glycoprotein, 1 µL of 10X Glycobuffer 4 and H20 (if necessary) to make a 10 µL total reaction volume.
  2. Add 1 µL Endo F3
  3. Incubate reaction at 37°C for 1 hour.

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