FAQ: Is it necessary to DNase treat my sgRNA synthesis reaction?

We recommend DNAse treating the sgRNA synthesis reaction with 2µl of the provided DNaseI (RNase-free) for 15 minutes at 37°C followed by spin column purification.  Removal of DNA template, and leftover NTPs and dNTPs, will result in a more reliable measurement of RNA yield as instruments that read A260 (such as a traditional UV spectrophotometer or NanoDrop™) will read both RNA and DNA and will not give a reliable concentration.  It is important to consider how leftover template, dNTPs and NTPs may affect downstream applications.