FAQ: Can acyclonucleotides be used in Sanger sequencing?

Acyclonucleotides are terminators that can substitute for ddNTPs in Sanger sequencing reactions when used with Therminator DNA polymerase (Gardner, A. and Jack, W. (2002) Acyclic and dideoxy terminator preferences denote divergent sugar recognition by archaeon and Taq DNA polymerases. Nuc. Acids Res. PMID: 11788725; Gardner, A.F., Joyce , C.M. and Jack, W.E. (2004) Comparative kinetics of nucleotide analog incorporation by Vent DNA polymerase. J. Biol. Chem. 279, 11834-11842. PMID: 14699133).